商品编号


LS002560



品牌


Worthington



公司


Worthington Biochemical Corporation



公司分类


Products



Size

1 gm

商品信息
PeroxidaseA soluble, dialyzed, lyophilized powder. RZ 1.0.Store at -20°C.≥85 units per mg dry weight
Source:
Horser
ADI
sh Roots
I.U.B.: 1.11.1.7
Peroxidase (HRP) is a hemoprotein catalyzing the oxidation by hydrogen peroxide of a number of substrates such as ascorbate, ferrocyanide, cytochrome c and the leuco form of many dyes. HRP has a molecular weight of 40 kDa and an optimum pH of 7.0. St
ABI
lity: HPOFF is stable for 9-12 months at 2-8°C. HPOD is stable 2 to 3 years at 2-8°C.
Unit Definition
: One
Worthington
Unit decomposes 1?mole of H
2
O
2
per minute at 25°C, pH 7.0 using aminoantipyrine and phenol.
Technical Note:
The RZ (Reinheitzahl) which is the absorbance ration, A
403
/A
275
, has been used as an indication of purity. However, Shannon
et al., JBC, 241
, 266 (1966) report that this ratio for the isozymes varies from 2.50 to 4.19. This, together with the influence exerted by buffer and pH, would seem to render questionable the preciseness of this ratio as a criterion of purity.
Numerous different methodologies are utilized for the determination of peroxidase activity. Listed below are some approximate conversions as determined by
Worthington
.
? 1
Worthington
unit = 4.6 o-dianisidine units previously used by
Worthington
? 1
Worthington
unit = 0.62 ABTS units (?mole of dye oxidized per minute, pH 6.0, 25°C, 1.7 mM dye)
? 1
Worthington
unit = 2 ABST units (?mole of dye oxidized per minute, pH 5.0, 25°C, 8.7 mM dye)
? 1
Worthington
unit = 0.5 guiacol units (?mole of guiacol oxidized per minute, pH 7.0, 25°C)
? 1
Worthington
unit = 0.5 pyrogallol to purpogallin unit (mg of product per 20 seconds, pH 6.0, 20°C)
? 1
Worthington
unit = 5 pyrogallol to purpogallin units (?mole of product per minute at pH 6.0, 30°C)