商品编号


LS001141



品牌


Worthington



公司


Worthington Biochemical Corporation



公司分类


Products



Size

1 gm

商品信息
Phosphatase, AcidA non-specific esterase partially purified to the 0.35-0.55 fraction by the method described by Singer, JBC, 174, 11 (1948). Also active as a lipase. A lyophilized powder.Store at -20°C.≥0.15 units per mg dry weight
Source:
Wheat Germ (Triticum vulgare)
I.U.B.: 3.1.3.2
Acid phosphatase is an esterase with broad activity at an optimal pH below 7.0. There are three isozymes, EI, EII, and EIII of similar molecular weight (55 kDa± 5 kDa). Their optimum pH's are 5.5, 4.5, and 4.0 respectively. Acid phosphatase activity was observed by Teller
Worthington
Library Archives
in 1954 in preparations of a wheat germ lipase described by Singer
JBC, 174
, 11, in 1948. Equivalent commercial preparations have been distributed labeled as lipase and acid phosphatase thus generating some confusion. Subsequent work has confirmed that the non-specific esterase activity of the wheat germ preparation may be measured both as lipase (triacetin as substrate) and phosphatase. The enzyme assay is based on the work of Brandenberger and Hanson (
Helv. Chim. Acta, 36
, 900, 1953) and Hofstee (
Arch. Biochem. Biophys., 51
, 239, 1954).
Unit Definition
: One Unit hydrolyzes one micromole of o-carboxyphenyl phosphate per minute at 25°C, pH 5.0.