商品编号


LS003926



品牌


Worthington



公司


Worthington Biochemical Corporation



公司分类


Products



Size

100 un

商品信息
Phosphodiesterase IPurified by the method of Williams, Sung and Laskowski, JBC, 236, 1130 (1961). Further treated to inactivate contaminating 5'-nucleotidase activity according to Sulkowski and Laskowski: BBA, 240, 443 (1961). Lyophilized in vials.Store at -20°C.REQUIRES SPECIAL SHIPPING: ICE PACK≥20 units per mg dry weight
Source:
Crotalus adamanteus Venom
I.U.B.: 3.1.4.1
Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-OH-terminated ribo- and deoxyribo-oligonucleotides. The enzyme has an optimal pH range of 9.8-10.4 and a molecular weight of 115 kDa. Phosphodiesterase is inhibited by reducing agents such as glutathione, cysteine and ascorbic acids. It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. The enzyme has an absolute requirement for Mg
2+
.
Unit Definition
: One Unit hydrolyzes one micromole of p-nitrophenyl thymidine-5-phosphate per minute at 25°C, pH 8.9.
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