商品编号


LS003602



品牌


Worthington



公司


Worthington Biochemical Corporation



公司分类


Products



Size

25 un

商品信息
Phosphodiesterase IIPrepared from the 1mM sodium pyrophosphate, pH 6.9, alumina gel eluate of Hilmoe: Biochem. Prep VIII (Meister, A., ed.), John Wiley & Sons, NY, NY, p. 105 (1961). Lyophilized in vials.Store at -20°C.REQUIRES SPECIAL SHIPPING: ICE PACK≥1.2 u/mg dw
Source:
Bovine Spleen
I.U.B.: 3.1.16.1
Spleen exonuclease (Phosphodiesterase II) excises 3'-phosphomononucleotides from oligonucleotides having a free OH terminus. The optimum pH for the enzyme is 5.5 using succinate and phosphate buffer and pH 6-7 with 0.1 M acetate buffer. The enzyme is assayed using a modification of the Hilmoe (
Biochem. Prep., VII
, (Meister, A., ed.), John Wiley and Sons, NY, 105, 1961) technique.
Unit Definition
: One Unit increases the absorbance at 260 nm by 0.200 in 30 minutes at 37°C, pH 6.5, with an RNA substrate.
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