商品编号


LS003113



品牌


Worthington



公司


Worthington Biochemical Corporation



公司分类


Products



Size

600 un

商品信息
Plasma Amine OxidaseSource: Bovine PlasmaChromatographically purified through step five of the procedure of Yamada, Y., and Yasunobu, K., JBC, 237, 1511 (1962). A lyophilized powder. (One IU = 4,330 Tabor Units).Store at -20°C.REQUIRES SPECIAL SHIPPING: ICE PACK≥17 Tabor units/mg dw
Source:
Bovine Plasma
I.U.B.: 1.4.3.21
Plasma amine oxidase (PAO) catalyzes the reaction:
RCH
2
NH
2
+ O
2
+ H
2
O → RCHO + NH
3
+ H
2
O
2
Bovine plasma amine oxidase has a molecular weight of 170 kDa and an optimum pH of 6.2 for spermine and 7.2 for spermidine. Amine oxidases are divided into two classes: the pyridoxal and copper containing enzyme to which plasma amine oxidase belongs, and the FAD-containing amino oxidases. Natural substrates include catecholamines, tryptamine derivatives and other physiologically active amines. Plasma amine oxidase is used in research requiring nitrogen group transfers. The molecule is composed of two identical polypeptide chains. There are two pyridoxal phosphates and two atoms of Cu
+
per molecule. Bovine plasma amine oxidase is inhibited by copper chelating agents, many carboxyl reagents such as cuprizone, hydroxylamine and cyanide. Benzoic acid and benzyl alcohol are both non-competitive inhibitors (K
I
= 30 and 34mM respectively). The assay for determination of amine oxidases employed at
Worthington
is essentially that of Tabor
et al., JBC, 208
, 645, (1954) with the reaction temperature reduced to 25°C.
St
ABI
lity/Storage:
Stable for 12 months at -20°C. Store at -20°C.
Unit Definition:
1 international unit oxidizes 1?mole of benzylamine per minute at 25°C, pH 7.2.
Technical Note:
1 I.U. equals 4,330 Tabor units. (T.U.)