商品编号


LS003608



品牌


Worthington



公司


Worthington Biochemical Corporation



公司分类


Products



Size

1 mg

商品信息
Protease, S. Aureus (Endoproteinase Glu-C)Chromatographically purified according to Drapeau, G., Boily, Y., and Houmard, J.: JBC, 247, 6720 (1972). A lyophilized powder.Store at 2-8°C.≥500 units per mg dry weight
Source:
Staph aureus V8
I.U.B.: 3.4.21.19
Protease
S. aureus
V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease
S. aureus
V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F
-
, Cl
-
, CH
3
COO
-
and NO
3
. Enzyme activity is determined by the casein digestion assay described by Drapeau (
Methods Enzymol., 45
, 469, 1976).
St
ABI
lity/Storage:
Autolysis occurs at temperatures greater than 40°C. The enzyme is fully active in
USP
0.2% SDS. Stable for 12 months at 2-8°C.
Unit Definition
: One Unit causes a change of 0.001 A
280
nm per minute at 37°C, pH 7.8 using casein as the substrate.
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