商品编号


LS01487



品牌


Worthington



公司


Worthington Biochemical Corporation



公司分类


Products



Size

500 ku

商品信息
Ribonuclease T1, Chromatographically Purified, Animal FreeHighly purified, microbial (non-mammalian) broad specificity RNase prepared with non-animal components. Supplied as a solution in 2.8M ammonium sulfate.Store at 2-8°C.REQUIRES SPECIAL SHIPPING: ICE PACK≥300,000 units per mg protein
Source:
Aspergillus oryzae
I.U.B: 3.1.27.3
Ribonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3'-phosphate residues (or inosine 3'-phosphate) and the 5'-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2', 3'-cyclic phosphates. It cleaves single-stranded RNA releasing oligonucleotides from the guanosine 3'-phosphate termini. The enzyme has a molecular weight of 11 kDa. The optimum pH is 7.5. RNase T1 is inhibited by Ag
+
, Zn
2+
, Cu
2+
, and Hg
2+
at 1 X 10
-3
M. The stimulatory effects of both histidine and EDTA are attributed to chelation of contaminating inhibitor cations. The enzyme assay is essentially the method of Egami
et al., Prog. in Nucleic Acid Res. and Molec. Biol., III
, 59 (1964) based upon the release of acid soluble oligonucleotides following the digestion of yeast RNA.
Uses:
Ribonuclease T1 has extensive applications in molecular cloning and DNA sequencing. Because of its specificity it has been a commonly used cleavage enzyme for the determination of structure, nearest neighbor frequencies, and RNA sequencing. The enzyme has further application in the preparation of nucleoside 2',3'-cyclic phosphates, the synthesis of oligonucleotides, and the removal of RNA from DNA preparations. The enzyme is also used as a non-mammalian source of RNase in various applications.
St
ABI
lity/Storage:
Stable 12-18 months at 2-8°C. Store at 2-8°C.
Unit Definition
: One Unit releases the equivalent of one A260 unit of acid-soluble products from yeast RNA in 15 minutes at 37°C, pH 7.5
Technical Note
: Some suppliers reference sequencing units; One sequencing unit is equivalent to 0.075
Worthington
unit.
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