商品编号


M0274S-300 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


PCR, qPCR & Amplification Technologies




300 units


Concentration

10,000 units/ml

商品信息

Description:
T7 DNA Polymerase catalyzes the replication of T7 phage DNA during infection. The protein dimer has two catalytic activities: DNA polymerase activity and strong 3?→ 5? exonuclease (1,2,3). The high fidelity and rapid extension rate of the enzyme make it particularly useful in copying long stretches of DNA template.
Highlights
Isolated from a recombinant source
Second strand synthesis in site-directed mutagenesis
Supplied with 10X Reaction Buffer
Gap filling reaction (no strand displacement)
Product Source
T7 DNA Polymerase consists of two subunits: T7 gene 5 protein (84 kilodaltons) and?
E.coli
thioredoxin (12 kilodaltons) (1,4-7). Each protein is cloned and overexpressed in a T7 expression system in?
E. coli?
(4).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
T7 DNA Polymerase Reaction Buffer
10X
Purified BSA
-20
10 mg/ml
Notes:
The high polymerization rate of the enzyme makes long incubations unnecessary.T7 DNA Polymerase is not suitable for DNA sequencing.