商品编号


M0247S-320,000 gel units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


DNA Modifying Enzymes



Concentration

2,000,000 gels units/ml


Size

320,000 gel units

商品信息

Description:
Micrococcal nuclease is derived from
Staphylococcus aureus
and is a relatively non-specific endo-exonuclease. It is purified from a recombinant
E. coli
strain that digests double-stranded, single-stranded, circular and linear nucleic acids. The enzyme is active in the pH range of 7.0 - 10.0, with optimal activity at pH 9.2 for both RNA and DNA substrates. Cleavage preferences have been observed at sites rich in adenylate, deoxyadenylate or thymidylate (1). Both DNA and RNA are degraded to 3? phosphomononucleotides and dinucleotides.
Micrococcal Nuclease works well with the SimpleChIP? Enzymatic Chromatin IP Kit (Agarose Beads) #9002 from Cell Signaling Technology. Please follow the link to see product information and protocols on their website.
Figure 1:
Digestion of 1 ?g of Lam
BD
a genomic DNA with Micrococcal Nuclease in a 3-fold dilution series. The amount of enzyme used in Lane 2 is defined as 1 gel unit. Lane M is the PCR
Marker
(
NEB
#N3234).
Highlights
Can be used for Chromatin Studies
Inactive until Ca2+ is added
Product Source
An
E. coli
strain containing a genetic fusion of the micrococcal nuclease gene (Gene ID: 3238436) and the gene coding for maltose binding protein, or MBP. The micrococcal nuclease is cleaved from the fusion protein and purified away from MBP.
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
Micrococcal Nuclease Buffer
10X
Purified BSA
-20
10 mg/ml
Notes:
This enzyme does not work in
NEB
uffer 1, 2, 3 or 4 due to the lack of Ca2+. Additional Ca2+ in
NEB
uffer only shows 10% activity. 1-5 mM Ca2+ is required for activity.The enzyme is active in the pH range 7-10 as long as salt concentration is less than 100 mM.Enzyme can be inactivated by addition of excess EGTA.