商品编号


H0260S



品牌


Biohelix



公司


BIO-HELIX Co., LTD.



公司分类


Helicases



Size

20 ?g


Concentration

0.5 mg/ml






商品信息



Description:


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E. coli
UvrD is a DNA helicase that uses the energy generated by the hydrolysis of nucleoside triphosphates to break the hydrogen bonds linking the two strands together in duplex DNA. It is active at 37?C.

SUPPLIED WITH:
10X Helicase Buffer M



Supplied by Users
: ATP




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Source:


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Purified from an
E. coli
strain that overexpresses
E. coli uvrD
gene.



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Reaction condition:


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1X Helicase Buffer M:
10 mM Dithiolthreitol
10 mM Magnesium Acetate
35 mM Tris-Acetate pH 7.5 @ 25°C

3 mM ATP.
Incubate at 37?C.


Notes:

E.coli UvrD helicase may require MutL and/or SSB for optimal performance in specific applications.

: MutL stimulates UvrD helicase activity more than tenfold by lo
ADI
ng it onto the DNA substrate (Mechanic LE, Frankel BA, Matson SW (2000) J Biol Chem 275: 38337?38346).

: MutL and SSB are required in Helicase-Dependent Amplification using E. coli UvrD (Myriam Vincent, Yan Xu, and Huimin Kong (2004) EMBO reports Vol. 5, 795 - 800).




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Storage Conditions:


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Storage Temperature:
-20?C


Concentration:

0.5 mg/ml

Storage Buffer:

20 mM Tris-HCl pH8.3
200 mM NaCl
1.5 mM EDTA
25 mM ?-Mercaptoethanol
50% Glycerol




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Quality Control:


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Quality Assurance Statement:

E. coli UvrD Helicase is purified free of contaminating endonucleases and exonucleases. Each lot is tested for DNA unwinding activity.




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