商品编号


H0240S



品牌


Biohelix



公司


BIO-HELIX Co., LTD.



公司分类


Helicases



Size

20 ?g


Concentration

0.5 mg/ml






商品信息



Description:


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Thermo
stable DNA Helicase is a DNA helicase that uses the energy generated by the hydrolysis of nucleoside triphosphates to break the hydrogen bonds linking the two strands together in duplex DNA. It is active in a temperature range of 45 to 65?C.

SUPPLIED WITH:
10X Annealing Buffer II
100 mM MgSO
4


Supplied by Users
: ATP




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Source:


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Purified from an
E. coli
strain that overexpresses the helicase gene isolated from a
Thermo
philic microorganism.



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Reaction condition:


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1X Annealing Buffer II:
10 mM KCl
20 mM Tris-HCl, pH 8.8 @ 25°C

Supplement with 4 mM MgSO
4
and 3 mM ATP.
Incubate at 45 - 65?C.





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Storage Conditions:


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Storage Temperature:
-20?C


Concentration:

0.5 mg/ml

Storage Buffer:

20 mM Tris-HCl pH7.4
200 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol



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Quality Control:


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Quality Assurance Statement:


Thermo
stable Helicase is purified free of contaminating endonucleases and exonucleases. Each lot is tested for DNA unwinding activity.




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