商品编号


1025



品牌


Chimerx



公司


Chimerx,Inc.



公司分类


DNA/RNA Modifying Enzymes




5,000 u

商品信息

Details


Source

Calf intestinal mucosa

Reagents Supplied

10X CIP Reaction Buffer

Applications


Features higher turnover rate and milder conditions of inactivation than bacterial enzyme (1)

Can be used to remove 5'-phosphates from DNA or RNA prior to 5'-end labeling (1)

Used to remove 5'-phosphates from linearized vector molecules to prevent self-ligation of the vector during cloning procedures (1)

Applicable to dephosphorylation of proteins


Unit Definition

One unit is the amount of enzyme required to hydrolyze 1 ?mol of p-nitrophenylphosphate to p-nitrophenol in 1 minute at 37°C (2)

Heat Inactivation

No

Concentration

20 - 25 units/μl

Storage Buffer

10 mM Tris-HCl (pH 8.0)
50 mM KCl
1 mM MgCl
2
?
0.1 mM ZnCl
2
?
50% (v/v) glycerol

Assay Conditions

See Certificate of Analysis Below?

Storage Conditions

Store at -20°C
Product shipped on dry ice

Downloads

Certificate of Analysis
Lot 2011002 PDF

MSD
S
MSD
S PDF
?

References

(1) Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition, pp. 5.72, Cold Spring Harbor, New York, NY
(2) Mosser, E., Boll, M., and Pfleiderer, G. (1980) Hoppe-Seyler's Z. Physiol. Chem. 361, 543-549