商品编号


4150



品牌


Tocris



公司


Bio-Techne Corporation



公司分类


Polymerases



Size

50 mg

商品信息

Description:
Selective reverse transcriptase inhibitor; antiretroviral

Chemical Name:
3'-Azido-3'-deoxythymidine

Purity:
≥99% (HPLC)


BIOLOG
ical Activity Technical Data Solubility Calculators Datasheets References


BIOLOG
ical Activity

Selective reverse transcriptase inhibitor. Exhibits 100-fold selectivity for HIV reverse transcriptase over DNA polymerase
α
. Suppresses HIV-1 replication and enhances cell vi
ABI
lity in HIV-1 infected T cells. Attenuates growth of multiple myeloma (MM) cells
in vitro
and reduces growth of MM tumor xenografts in mice. Orally bioavailable and brain penetrant. Antiretroviral agent. Also decreases CRISPR-mediated homology directed repair (HDR) efficiency.



Licensing Information

Sold for research purposes under agreement from Viiv.



Technical Data



M. Wt

267.24



Formula

C
10
H
13
N
5
O
4




Storage

Store at -20°C



Purity

≥99% (HPLC)



CAS Number

30516-87-1



PubChem ID

35370



InChI Key

HBOMLICNUCNMMY-XLPZGREQSA-N



Smiles

O=C1N([C@@H]2O[C@H](CO)[C@@H](N=[N+]=[N-])C2)C=C(C)C(N1)=O



The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All
Tocris
products are intended for laboratory research use only.



Solubility Data




Solvent

Max Conc. mg/mL

Max Conc. mM



Solubility



DMSO

26.72

100



water

13.36

50





Preparing Stock Solutions

The following data is based on the
product
molecular weight
267.24
. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.



Concentration / Solvent Volume / Mass

1 mg

5 mg

10 mg



1 mM

3.74 mL

18.71 mL

37.42 mL



5 mM

0.75 mL

3.74 mL

7.48 mL



10 mM

0.37 mL

1.87 mL

3.74 mL



50 mM

0.07 mL

0.37 mL

0.75 mL






Molarity Calculator






Molarity Calculator

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Reconstitution Calculator






Reconstitution Calculator



The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.



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ul



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ng



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Dilution Calculator






Dilution Calculator

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Product Datasheets


Certificate of Analysis / Product Datasheet


Safety Datasheet

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References

References are publications that support the products'
BIOLOG
ical activity.


Mitsuya

et al
(1985) 3'-Azido-3'-deoxythymidine (BW A509U): an antiviral agent that inhibits the infectivity and cytopathic effect of human T-lymphotropic virus type III/lymphadenopathy-associated virus in vitro. Proc.Natl.Acad.Sci.U.S.A.
82
7096 PMID: 2413459


Furman

et al
(1986) Phosphorylation of 3'-azido-3'-deoxythymidine and selective interaction of the 5'-triphosphate with human immunodeficiency virus reverse transcriptase. Proc.Natl.Acad.Sci.U.S.A.
83
8333 PMID: 2430286


Broder
(2010) The development of antiretroviral therapy and its impact on the HIV-1/AIDS pandemic. Antiviral Res.
85
1 PMID: 20018391


Pereira

et al
(2013) Azidothymidine is effective against human multiple myeloma: a new use for an old drug? Anticancer Agents Med.Chem.
13
186 PMID: 22931421


Yu

et al
(2015) Small molecules enhance CRISPR genome editing in pluripotent stem cells. Cell Stem Cell
16
142 PMID: 25658371




Keywords: Azidothymidine, supplier, selective, reverse, transcriptase, inhibitors, inhibits, antiretroviral, HIV, human, immunodeficiency, virus, dna, rna, polymerase, CRISPR, Cas9, HDR, homology, directed, repair, RNA/DNA, Polymerase,
Tocris
Bioscience