商品编号


6156



品牌


Tocris



公司


Bio-Techne Corporation



公司分类


Ligases



Size

10 mg

商品信息

Description:
High-affinity inhibitor of VHL

Chemical Name:
(2
S
,4
R
)-1-((
S
)-2-(1-cyanocyclopropanecarboxamido)-3,3-dimethylbutanoyl)-4-hydroxy-
N
-(4-(4-methylthiazol-5-yl)benzyl)pyrrolidine-2-carboxamide

Purity:
≥98% (HPLC)


BIOLOG
ical Activity Technical Data Solubility Calculators Datasheets References


BIOLOG
ical Activity

High-affinity inhibitor of E3 ubiquitin ligase VHL (K
d
= 80-90 nM). Blocks interaction between VHL and HIF-α downstream of HIF-α hydroxylation, initiating hypoxic response. Results in time- and concentration-dependent accumulation of hydroxylated HIF-α, and upregulates mRNA and protein levels of HIF target genes. Cell permeable. Negative control
cis
VH 298 also available.



Licensing Information

Sold under licence from the University of Dundee



External Portal Information

Chemicalprobes.org
is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of VH 298 is reviewed on the Chemical Probes website.



Technical Data



M. Wt

523.65



Formula

C
27
H
33
N
5
O
4
S



Storage

Store at -20°C



Purity

≥98% (HPLC)



PubChem ID

122199236



InChI Key

NDVQUNZCNAMROD-RZUBCFFCSA-N



Smiles

O=C(N[C@@H](C(C)(C)C)C(N1C[C@@H](C[C@H]1C(NCC2=CC=C(C3=C(N=CS3)C)C=C2)=O)O)=O)C4(C#N)CC4



The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All
Tocris
products are intended for laboratory research use only.



Solubility Data




Solvent

Max Conc. mg/mL

Max Conc. mM



Solubility



DMSO

52.37

100



ethanol

52.37

100





Preparing Stock Solutions

The following data is based on the
product
molecular weight
523.65
. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.



Concentration / Solvent Volume / Mass

1 mg

5 mg

10 mg



1 mM

1.91 mL

9.55 mL

19.1 mL



5 mM

0.38 mL

1.91 mL

3.82 mL



10 mM

0.19 mL

0.95 mL

1.91 mL



50 mM

0.04 mL

0.19 mL

0.38 mL






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Reconstitution Calculator



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Product Datasheets


Certificate of Analysis / Product Datasheet


Safety Datasheet

Select another language:



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View SDS





References

References are publications that support the products'
BIOLOG
ical activity.


Frost

et al
(2015) Potent and selective chemical probe of hypoxic signalling downstream of HIF-α hydroxylation via VHL inhibition. Nat.Commun.
7
13312 PMID: 27811928




Keywords: VH 298, supplier, Inhibitors, Inhibition, VH298, VHL, HIF-alpha, HIF-a, HIF-α, cisVH298, von, Hippel-Lindau, protein, E3, ubiquitin, ligase, Ubiquitin, E3, Ligases,
Tocris
Bioscience