商品编号


EG-1004



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


DNA Modifying Enzymes



Size

10,000 Units

商品信息



Description


T4 Polynucleotide Kinase (PNK) catalyzes the transfer of the terminal gamma-phosphate from ATP to the 5′-OH group of single- and double-stranded DNA, RNA, oligonucleotides and nucleoside 3′-monophosphate molecules. In the presence of ADP T4 Polynucleotide Kinase also exhibits 5'-phosphatase activity and catalyzes the exchange of phosphate groups between 5'-P-oligo-polynucleotides and ATP.




Applications



End-labeling DNA or RNA for probes and DNA sequencing

Addition of 5?-phosphates to oligonucleotides to allow subsequent ligation

Removal of 3?-phosphoryl groups





Source

A recombinant
E. coli
strain carrying T4 phage
pse
T gene for polynucleotide kinase.



Unit Definition

One unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of [
32
P] (ATP donor) in 30 minutes at 37°C in 1X T4 Polynucleotide Kinase Reaction Buffer.



Components



T4 Polynucleotide Kinase:
10,000 U/ml in 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1 μM ATP, pH 7.4 @ 25°C

10X Reaction Buffer
: 700 mM Tris-HCl, 100 mM MgCl
2
, 50 mM DTT, pH 7.6 @ 25°C





Quality Control

The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests. Functionally tested for labeling 5'-termini of DNA.



Storage Condition


-20 °C