商品编号


EG-1005



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


DNA Modifying Enzymes



Size

1,000 Units

商品信息



Description


Human apurinic/apyrimidinic (AP) endonuclease, APE 1, also known as HAP 1 or Ref-1, is a homolog of
Escherichia coli

Exonuclease III
. APE1 acts as an AP lyase by hydrolyzing the phosphodiester backbone at the 5′ end of an apurinic (AP) site, generating a single-strand DNA break in the DNA duplex and leaving 3′-hydroxyl and 5′-deoxyribose phosphate termini. It is reported that APE 1 may also exhibit weak DNA 3′-diesterase, 3′ to 5′ exonuclease and RNase H activities.




Applications



Single cell gel electrophoresis (Comet assay)

Alkaline elution

Alkaline unwinding

Modified nick translation





Source

An
E. coli
strain which carries the cloned human APE 1 gene.



Unit Definition

One unit is defined as the amount of enzyme required to cleave 20 pmol of a 34-mer oligonucleotide duplex contain-ing a single AP site in 1 hour at 37°C



Components



APE 1
: 10,000 units/ml in 10 mM Tris-HCl, pH 8.0 @ 25°C, 50 mM NaCl, 1 mM DTT, 0.05 mM EDTA, 200 μg/ml BSA, 50% Glycerol


10X Reaction Buffer
: 200 mM Tris-Acetate, 500 mM Potassium Acetate, pH 7.9 @ 25°C, 10 mM Dithiothreitol, 100 mM Magnesium Acetate.





Quality Control

The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests. Functional test.



Storage Condition


-20 °C