商品编号


EG-158



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


DNA Modifying Enzymes



Size

2,500 Units

商品信息



Description


T4 Phage β-glucosyltransferase is a DNA-modifying enzyme encoded by bacteriophage T4 that specifically transfers the glucose moiety of uridine diphosphoglucose (UDP-Glc) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA, resulting in the formation of beta-glucosyl-5-hydroxymethylcytosine.




Applications



Locus specific immunodetection detection of 5-hmC DNA

Enrichment of 5-hmC containing DNA

Labeling of 5-hmC residues using a r
ADI
oactive UDP-glucose donor





Source

An E. coli strain that carries the cloned bgt gene from bacteriophage T4



Unit Definition


One unit is the amount of T4 Beta-glucosyltransferase required to protect 0.5 ?g of fully 5-hydroxymethylated 1 kb PCR fragment from cleavage by EcoR 1.




Components



T4 Beta-glucosyltransferase:
10,000 units/ml in 10 mM Tris (pH 7.5 at 25°C), 500 mM NaCl, 0.1 mM EDTA, 1 mM DTT and 50% (v/v) glycerol.

10X Reaction Buffer
: 330 mM Tris-acetate (pH 7.5), 660 mM potassium acetate, 100 mM magnesium acetate, and 5 mM DTT.





Quality Control


The absence of exonuclease, endonuclease, and RNase is confirmed by appropriate quality test.




Storage Condition


-20 °C