商品编号


EG-28



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


Ligases




100 μg

商品信息



Description

E coli
BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'-adenylate and transfers the biotin group to the amino group of a lysine residue present within biotin-accepting proteins, such as biotin carboxy carrier protein (BCCP) or proteins fused to a 15 amino acid peptide termed AviTag (GLNDIFEAQKIEWHE)



Applications


Covalently biotin-labeling of acceptor proteins fused to AviTag in vitro for detection and purification assays




Source

Purified from a recombinant
E. coli
strain carrying the E. coli birA protein ligase gene (NP_418404)



Components

BirA Biotin Ligase
, 1 μg/μl in storage buffer: 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT, 50% glycerol, pH 7.5 @ 25°C.



Activity

1 μg is sufficient to transfer biotin into 50μg target protein at room temperature or at 4°C overnight (>95%).



Quality Control



The absence of protease was confirmed by appropriate quality tests.

Functionally tested for the capacity to label proteins with biotin
in vitro
.





Storage

-20°C.