商品编号


EG-147



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


Ligases




50 ?g

商品信息



Description


Thermo
stable ATP independent RNA Ligase 2 specifically ligates the preadenylated 5? end of ssDNA or RNA to the 3? OH end of RNA at 60–65°C. The enzyme does not require ATP for ligation but requires pre-adenylated linkers.




Applications

Ligate a 5? pre-adenylated 3?-blocked DNA or RNA sequence tag to the 3?-OH of RNA and ssDNA.



Source

Purified from a recombinant
E. coli
strain carrying a
Thermo
stable
archaeal
RNA Ligase



Components



Thermo
stable ATP-independent RNA Ligase
, 1 mg/ml in storage buffer: 50 mM KCl, 10 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol, pH 7.5 @ 25°C

10 X
Thermo
stable ATP-independent RNA Ligase Buffer:
100 mM Bis-Tris-Propane-HCl, 100 mM MgCl2, 10 mM DTT, pH 7 @ 25°C





Activity


1 ?g protein will ligate at least 16 pmol of pre-adenylated donor substrate RNA to 10 pmol of acceptor substrate (e.g. 17mer ssDNA) in a total reaction volume of 200 μl in 1 hour at 65°C.




Quality Control


The absence of endo-, exodeoxyribonucleases, phosphatase and ribonucleases confirmed by appropriate quality tests.




Storage

-20°C.