商品编号


EG-148



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


Ligases




500 Units

商品信息



Description


Thermo
stable RNA Ligase I is an ATP-dependent ligase that catalyzes intra- and intermolecular ligation of single-stranded RNA (ssRNA) molecules having a 5?-phosphate and a 3?-hydroxyl group at 60 ~65°C. This ligase also ligatesssDNA molecules.




Applications



RNA-Ligase-Mediated Rapid Amplification of
CDN
A Ends (RLM-RACE).

Ligation of ssRNA molecules.

Ligation of adaptors to ssRNA or ssDNA.

5?-ligation tagging of RNA molecules.





Source

Purified from a recombinant
E. coli
strain carrying a
Thermo
vibrio ammonificans
RNA Ligase 1 gene



Components



Thermo
stable RNA Ligase 1
, 100 U/?l in storage buffer: 50 mM KCl, 10 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol, pH 7.5 @ 25°C

10 X
Thermo
stable RNA Ligase 1 Buffer:
100 mM Bis-Tris-Propane-HCl, 100 mM MgCl2, 10 mM DTT, 0.5 mM ATP, pH 7 @ 25°C





Unit Definition


1 unit of
Thermo
stable RNA Ligase catalyzes the conversion of 10 pmole of a linear 5?-phosphorylated poly(A)18 to a phosphatase resistant form in one hour at 60°C under standard assay conditions




Quality Control


The absence of endo-, exodeoxyribonucleases, phosphatase and ribonucleases confirmed by appropriate quality tests.




Storage

-20°C.