商品编号


EG-1054



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


Polymerases




5,000 Units

商品信息



Description

DNA Polymerase I (
E. coli
) is a mesophilic DNA polymerase that exhibits 5′-3′ DNA synthesis in addition to both 3′→ 5′ and 5′→ 3′ exonuclease activities. The combination of DNA synthesis and 5′→ 3′ nuclease characteristics enable nick-translation during DNA synthesis.



Applications



Nick translation of DNA in conjunction with DNase to obtain probes with a high specific activity

Second strand synthesis of
CDN
A in conjunction with RNase H





Source

A recombinant
E. coli
strain carrying
E coli
PolA gene.



Unit Definition

1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37°C.



Components



DNA Polymerase l:
10,000 U/ml in
25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 @ 25°C

10X Reaction Buffer
: 500 mM NaCl, 100 mM Tris-HCl, 100 mM MgCl
2
, 10 mM DTT, pH 7.9 @ 25°C





Quality Control

The absence of endodeoxyribonucleases confirmed by appropriate quality test.



Storage Condition

-20 °C