商品编号


EG-1055



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


Polymerases




250 Units

商品信息



Description

Klenow Fragment is a truncated verson of
E coli
DNA Polymerase I. The enzyme exhibits 5'→3' polymerase (DNA synthesis) and proofre
ADI
ng (3′→5′) nuclease activities, but lacks 5'→3' exonuclease activity.



Applications



Removeal of 3? overhangs to form blunt ends

Random-primed DNA labeling

Labeling by fill-in 5'-overhangs of dsDNA to form blunt ends

DNA sequencing by the Sanger method

Site-specific mutagenesis of DNA with synthetic oligonucleotides

Second strand synthesis of
CDN
A





Source

A recombinant
E. coli
strain carrying the Klenow Fragment of
E coli
PolA gene.



Unit Definition

1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37°C.



Components



Klenow Fragment:
5,000 U/ml in 25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 @ 25°C

10X Reaction Buffer
: 500 mM NaCl, 100 mM Tris-HCl, 100 mM MgCl
2
, 10 mM DTT, pH 7.9 @ 25°C





Quality Control



The absence of endodeoxyribonucleases confirmed by appropriate quality test.

Functionally tested for fill in of 5'-overhanging DNA termini and for random primed DNA labeling.





Storage Condition

-20 °C