商品编号


EG-1062



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


Polymerases




10,000 Units

商品信息



Description


Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of single or double stranded DNA molecules. The presence of 1 mM Co
2+
stimulates the tailing of the 3'-ends of DNA fragments.




Applications



Addition of homopolymer tails to the 3' ends of DNA

Labeling the 3' ends of DNA with modified nucleotides (e.g., ddNTP, DIG-dUTP)

TUNEL assay (
in situ
localization of apoptosis)

TdT dependent PCR





Source

A recombinant
E. coli
strain carrying an engineered human terminal deoxynucleotidyl transferase gene



Unit Definition


1 unit is defined as the amount of polymerase required to convert 1 nmol of dATPs into acid insoluble material in 1 hour at 37°C.






Components



Terminal deoxynucleotidyl Transferase:
20,000 U/ml in
50 mM KPO
4
, 100 mM NaCl,
1.0 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1% Triton X-100, pH 7.3 @ 25°C

10X Reaction Buffer
: 200 mM Tris-Acetate, 500 mM Potassium Acetate, 100 mM Magnesium Acetate, 10 mM DTT, pH 7.9 @ 25°C

2.5 mM CoCl
2





Storage Condition

-20 °C