商品编号


EG-1083



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


Polymerases




4,000 Units

商品信息



Description


Recombinant Bst DNA Polymerase (large fragment) has 5’-> 3’ polymerase activity, but lacks 5’->3’ exonuclease activity. This enzyme can be used for and rapid amplification of trace amounts of DNA templates for sequencing.




Applications



DNA sequencing through high GC regions

Rapid amplification of trace amounts of DNA templates

Next Generation sequencing





Source

A recombinant
E. coli
strain carrying the large fragment of DNA polymerase gene from Bacillus stearo
Thermo
philus



Unit Definition

One unit is defined as the amount of polymerase required to catalyze the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 65°C.



Components



Bst DNA Polymerase:
8,000 U/ml in 10 mM Tris-HCl, 50 mM KCl, 1.0 mM Dithiothreitol, 0.1 mM EDTA, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25°C

10X Reaction Buffer
: 200 mM Tris-HCl, 100 mM (NH
4
)
2
SO
4
, 100 mM KCl, 20 mM MgSO
4
, and 1.0 % Triton X-100, pH 8.8 @ 25°C





Quality Control

The absence of endonuclease, nicking activity and

exonuclease

activity is confirmed by appropriate quality tests.



Storage Condition


-20 °C