商品编号


30061-3



品牌


Lucigen



公司


Lucigen



公司分类


Modifying Enzymes



Size

5,000 U

商品信息


Recombinant T4 Polynucleotide Kinase (T4 PNK).

Applications include end-labeling and insert preparation for cloning.




T4 Polynucleotide Kinase catalyzes the transfer and exchange of gamma-phosphate of ATP to the 5?-hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3?-monophosphates.


Source:
Purified from a strain of
E. coli
that carries a T4 Polynucleotide Kinase expression plasmid.


Applications:


End-labeling DNA for probes and DNA sequencing (1)

Addition of 5?-phosphates to oligonucleotides to allow subsequent ligation (2)



Heat inactivation:
70°C for 15 minutes.


Storage:
Enzyme and buffer should be stored at -20°C. Enzyme is stable for 12 months if handled properly.


Additional Information:


Ammonium ions are potent inhibitors of T4 Polynucleotide Kinase.? DNA should not be precipitated from or dissolved in buffers containing ammonium salts.

Protruding 5’-overhangs are more efficiently phosphorylated than blunt- or recessed 5’ overhangs.



References:


Sambrook, J. and Russell, W. (2001)
Molecular Cloning: A Laboratory Manual
, (3rd ed.), 9.55-9.56, 9.68-9.69.

Sambrook, J. and Russell, W. (2001)
Molecular Cloning: A Laboratory Manual
, (3rd ed.), 13.16-13.17.