商品编号


30221-1



品牌


Lucigen



公司


Lucigen



公司分类


Polymerases




2,000 U


商品信息



Excellent strand displacement, fidelity and speed.





phi29 DNA Polymerase is a highly processive DNA polymerase with a powerful strand displacement activity and a 3′→ 5′ proofre
ADI
ng exonuclease function. The enzyme is capable of up to 70,000 insertions per binding event.

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An example of the savings you can expect.



Specifications

Unit Definition:
1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30°C.

Source:
A recombinant
E. coli
strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29.



Unit Concentration

10,000 U/mL



Purity (SDS-PAGE)

>99%



SS Exonuclease

Functional



E. coli
16S rDNA Contamination

100 U <10 copies



Endonuclease

100 U <10% converted



Storage

-20&°C



ORDER INFORMATION
Supplied at 10,000 U/mL in 10 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.5% Tween-20, 0.5% NP-40, 50% glycerol, pH 7.4 @ 25°C. Also supplied is 10X phi29 DNA Polymerase Buffer composed of 500 mM Tris-HCl, 100 mM (NH
4
)
2
SO
4
, 40 mM Dithiothreitol, 100 mM MgCl
2
, pH 7.5 @ 25°C. 10,000 U is supplied as 5 × 2,000 U.