商品编号


BPR-205



品牌


MCLab



公司


MCLAB.Inc



公司分类


Mesophilic DNA Polymerases



Size

20,000 units, 8 U/?l

商品信息

For replicating difficult templates in various applications.


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Description:
rBst DNA Polymerase is a product of DNA poll gene of the
Thermo
philic bacterium
Bacillus stearo
Thermo
philus
(Bst). ?The enzyme contains 5? -> 3? polymerase activity, but lacks the 3? -> 5? exonuclease activity. It also has an optimal activity at 65°C and can be used to synthesize DNA in regions containing template secondary structure or high GCs where other non-
Thermo
stable DNA polymerases may fail in sequencing. Therefore, rBst DNA Polymerase is useful in replicating difficult templates in various applications.
Application:
- Isothermal DNA amplification by the method of:
· loop-mediated isothermal amplification (LAMP)
· whole genome amplification (WGA)
· ramification amplification (RAM)
- Random-primed DNA labeling
- Labeling by fill-in 5'-overhangs of dsDNA
Source:
An
E. coli
strain that contains the gene from
Bacillus stearo
Thermo
philus
.
Supplied in:
10 mM Tris-HCl
50 mM KCl
1.0 mM DTT
0.1 mM EDTA
0.1% Triton X-100
50% Glycerol
pH 7.5 @ 25°C
Supplied with:
10X PCR buffer II
10x PCR Buffer ll:
200 mM Tris-HCl
100 mM Ammonium Sulfate
100 mM KCl
20 mM MgSO
4
1.0% Triton X-100
pH 8.8 @ 25°C
Unit Definition:
1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°C.
Recommended Storage Condition:
-20°C
Reference:
Kiefer, et al. Structure 15 January 1997. 5, 95-108.