商品编号


FC-121-3003



品牌


宜曼达(Illumina)



公司


Illumina, Inc.



公司分类


Library Preparation Kits


商品信息

Product Highlights:
TruSeq DNA PCR-Free Library Preparation Kits provide simple, all-inclusive library preparation for whole-genome sequencing applications. Researchers can sequence a wide variety of organisms, from small genomes such as bacteria to human whole-genomes. The kits offer:


Shortened gel-free workflows that remove the need for PCR

ABI
lity to sequence the most challenging regions

Improved genome coverage to identify the greatest number of variants


Sequence the most challenging regions

TruSeq DNA PCR-free kits offer superior coverage of areas which are tr
ADI
tionally difficult to sequence such as high GC-rich regions, promoters, and repetitive content.

The kits are tunable to a variety of read lengths and are supported on all
Illumina
sequencing instruments. This permits the researcher to tailor each run to the needs of the experiment.

Detect the greatest number of variants

PCR-free means reduced library bias and gaps. The result is unsurpassed data quality. This enables you to detect the greatest number of variants. Excellent genome coverage means your results have the lowest and smallest number of gaps and enhanced coverage of high G/C rich regions.


Use PCR-free for faster protocols

Removing PCR creates a faster protocol and superior data quality. Bead-based size selection shortens the workflow. In tandem with
Illumina
sequencing systems, the TruSeq DNA PCR-Free Library Preparation Kit provides a range of enhancements to the industry's most widely adopted library preparation workflow.

Access flex
IBL
e throughput options

Kits include reagents, sample purification beads, and indexes, with two options for flexibility:


TruSeq DNA PCR-Free LT Library Preparation Kits support 24-plex manual processing for low-throughput studies.

TruSeq DNA PCR-Free HT Library Preparation Kits are 96-plex for high-throughput studies, and can be automated on liquid handling robots (or processed manually).


Find an up-to-date list of automation vendors with robotic systems that support the HT library preparation kits
Specifications:
Input Quantity
0.1 – 1 ug high-quality purified total RNA from blood
Content Specifications
Captures coding RNA plus multiple forms of non-coding RNA
Mechanism of Action
Bead-based rRNA depletion,
CDN
A synthesis, and PCR
Multiplexing
Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kit version: Prepare 96 uniquely indexed samples
System Compatibility
NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 500,HiSeq 2500,NextSeq 550,HiSeq 3000,HiSeq 1000
Automation Cap
ABI
lity
Liquid Handling Robots
Variant Class
Transcript Variants,Single Nucleotide Polymorphisms (SNPs),Gene Fusions
Species Category
Rat,Mouse
Method
Whole-Transcriptome Sequencing
Technology
Sequencing