商品编号


J5110



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


E3 Ubiquitin Ligases



Size

1 Kit

商品信息

This kit is designated for monitoring CHIP-mediated ubiquitination of user-supplied misfolded proteins. CHIP is an E3 ubiquitin ligase that directly or via Hsp70 interacts with misfolded proteins and promotes their polyubiquitination, which targets misfolded proteins for proteasomal degradation.

Additional Information






Product Name:

Misfolded Protein Ubiquitination Kit



Also Known As:

N/A



Catalog No.:

J5110



Size:

1 Kit



Molecular Weight:

N/A



Species:

N/A



Source:

N/A



Stock:

Enzyme stocked in 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol



Concentration:

See tube label



Quality Assurance:

Enzyme >95% by SDS-PAGE



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF datasheet
,
MSD
S



NCBI RefSeq:

N/A



Image(s):

No



Shipping Method:

Dry ice shipping



References:

N/A




Details

* This kit is enough for setting up 20 x 20 ?l reactions.

Description
This kit is designated for monitoring CHIP-mediated ubiquitination of user-supplied misfolded proteins. CHIP is an E3 ubiquitin ligase that directly or via Hsp70 interacts with misfolded proteins and promotes their polyubiquitination, which targets misfolded proteins for proteasomal degradation.

Components
? 10x Human Ubiquitin (500 uM)..............................................40 ?l ? 20x UBE1 (E1, 2 uM))............................................................20 ?l
? 10x 6xHis-UbE2D3 (E2, 20 uM)............................................40 ?l ? 20x 6XHis-CHIP (E3, 40 uM))................................................20 ?l
? 10x Hsp70 (20 uM)................................................................40 ?l ? 5x Ubiquitination Buffer.......................................................150 ?l
? 10x ATP (40 mM)...................................................................60 ?l