商品编号


RS-122-2103



品牌


宜曼达(Illumina)



公司


Illumina, Inc.



公司分类


Library Preparation Kits


商品信息

Product Highlights:
The TruSeq Stranded mRNA Library Prep Kit offers a streamlined, cost-efficient, and scalable solution for coding transcriptome analysis. It is compat
IBL
e with a wide range of samples.

Precise and Accurate

Get precise measurement of mRNA strand orientation for detection of antisense transcription, enhanced transcript annotation, and increased alignment efficiency. High coverage uniformity enhances the discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.



Cost-Efficient

Stranded information identifies from which of the 2 DNA strands a given RNA transcript was derived. This information provides increased confidence in transcript annotation, particularly for nonhuman samples. Identifying strand origin increases the percentage of reads that align, reducing sequencing costs per sample.

Scalable

The kit enables robust interrogation of both standard and low-quality samples, and workflows compat
IBL
e with a wide range of study designs.

Find an up-to-date list of high-throughput automation vendors with robotic systems compat
IBL
e with this library preparation kit
Specifications:
Assay Time
~10.5 hours
Hands-On Time
~4.5 hours
Input Quantity
0.1 – 1 ug total RNA or 10 - 100 ng previously isolated mRNA (from species with polyA tails)
Content Specifications
Captures the coding transcriptome with strand information
Mechanism of Action
Oligo-dT beads capture polyA tails
System Compatibility
NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Species Category
Other,Mammalian,Bovine,Mouse,Human,Rat
Variant Class
Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants
Technology
Sequencing
Method
mRNA Sequencing
Automation Cap
ABI
lity
Liquid Handling Robots