商品编号


NP-202-1001



品牌


宜曼达(Illumina)



公司


Illumina, Inc.



公司分类


Library Preparation Kits


商品信息

Product Highlights:
The TruSeq Stranded mRNA Library Prep Kit for NeoPrep offers a streamlined, cost-efficient, and scalable solution for coding transcriptome analysis. It enables robust interrogation of both standard and low-quality samples.


Simplified and seamless workflow solution
– Generate libraries with minimal hands-on time

Unparalleled performance and reproducibility
– Interrogate gene expression with TruSeq Stranded mRNA coverage and quality, and reduced user vari
ABI
lity

Low input requirement
– Begin library prep with as little as 25 ng total RNA


Precise and Accurate

Get precise measurement of mRNA strand orientation for detection of antisense transcription, enhanced transcript annotation, and increased alignment efficiency. High coverage uniformity enhances the discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.


Cost-Efficient

Stranded information identifies from which of the 2 DNA strands a given RNA transcript was derived. This information provides increased confidence in transcript annotation, particularly for nonhuman samples. Identifying strand origin increases the percentage of reads that align, reducing sequencing costs per sample.

NeoPrep System enhances TruSeq Stranded mRNA performance

The NeoPrep System enhances TruSeq Stranded mRNA performance by providing high-quality reproduc
IBL
e results, even with low input amounts of RNA. Digital microfluidics technology precisely manipulates droplets that perform the library prep workflow, within the tightly controlled environment of the NeoPrep library card.

A simple, intuitive workflow delivers 16 libraries, eliminating most manual steps, and reducing hands-on time from ~4.5 hours to just 30 minutes. In addition, digital microfluidics requires less RNA input, enabling excellent performance from as little as 25 ng of total RNA.
Specifications:
Assay Time
~10.5 hours
Hands-On Time
~30 minutes
Input Quantity
25 to 100 ng total RNA from species with polyA tails
Content Specifications
Captures the coding transcriptome with strand information
Mechanism of Action
Oligo-dT beads capture polyA tails
Multiplexing
Up to 24-plex pooling with additional NeoPrep runs
Sample Throughput
Up to 16 samples per NeoPrep library card
System Compatibility
Genome Analyzer IIx,HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,NeoPrep,HiSeq 1500,NextSeq 500,HiSeq 2500,HiSeq 4000
Species Category
Other,Mammalian,Bovine,Mouse,Human,Rat
Variant Class
Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants
Technology
Sequencing
Method
mRNA Sequencing
Automation Cap
ABI
lity
NeoPrep Digital Microfluidics