商品编号


97-101



品牌


Expressionsystems



公司


Expression Systems, LLC



公司分类


Reagents



Size

1 Ea

商品信息
Baculovirus Titering Kit
Expression Systems’ Baculovirus Titering Kit
facilitates accurate determination of infectious baculovirus titers in less than 24 hours. The assay is based on detection of the baculovirus gp64 fusion protein which is expressed on the surface of infected insect cells within six hours of infection. The kit contains gp64-PE antibody and a baculovirus control of known titer.
Description:
Staining of cells with a fluorescently labeled anti-gp64 antibody allows for identification of infected insect cells. By inoculating cultures with a series of log dilutions of virus, and staining the cultures 18–20 hours post inoculation, the ratio of infected to uninfected insect cells can be determined by flow cytometry. Statistical analysis of the percentage of infected cells in the virus dilution series enables accurate infectious titer determination.

Alternative assays that also claim results in under 24 hours (such as particle counting instruments and qPCR) are indeed fast but do not deliver true infectious titer values. In contrast to the plaque assay, the culturing conditions employed for the flow cytometric assay closely reflect actual culturing conditions utilized for expression cultures, resulting in a more relevant titer value in a fraction of the time. Provided access to a flow cytometer equipped to handle 96 well plates, the flow cytometric assay is easily adapted to high throughput processes.


Reproduc
IBL
e and accurate titer results within 24 hours

Measures infectious virus

S
USP
ension culture methodology simulates actual production conditions

Works with any flow cytometer capable of re
ADI
ng samples from 96-well plates

Flow cytometric data collection removes the operator error of counting plaques

gp64-PE antibody can be used alone to monitor infection kinetics of baculovirus in insect cell culture



Contents:

gp64-PE (R-PHYCOERYTHRIN (R-PE)-CONJUGATED MOUSE ANTI-BACULOVIRUS MONOCLONAL ANTIBODY)

Clone: AcV1

Antibody Composition: Mouse IgG2A

Storage Buffer: Phosphate buffered saline (PBS) containing 0.05% BSA

and 0.09% sodium azide.

Recombinant Baculovirus Control

BestBac 2.0 co-transfected with empty pVL1393 produced in Sf9 cells
Specification:



Media type
ESF 921 or ESF AF



Platform compatibility
Works with infectious baculovirus produced by most methods



Shipping condition
Baculovirus Titering Kit ships on cold packs, gp64-PE ships ambient



Storage condition
2-8?C, protect from light



Use-by date
Six months from Date of Manufacture for Baculovirus Control




Recommended Storage Condition
2–8? C