商品编号


EC02T110



品牌


Lucigen



公司


Lucigen



公司分类


CRISPR, Large or Difficult Fragment Cloning




10 x 100 ?l

商品信息

Standard cloning for large inserts and T1 phage resistance

Avoid phage contamination with tonA mutation for resistance to bacteriophages T1 and T5.

Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA.

Generate clones with induc
IBL
e copy number using CopyControl? vectors.

Achieve high transformation efficiencies: >1 × 10
10
cfu/?g pUC19 DNA.




Applications

Construction of induc
IBL
e-copy-number genomic libraries using the CopyControl? Cloning System, with clones that are resistant to contaminating phage T1 and T5.

Phage T1-Resistant TransforMax? EPI300?-T1
R
E. coli
have all the benefits of the highly versatile TransforMax EPI300?
E. coli
competent cells with the addition of being resistant to bacteriophages T1 and T5 (
tonA
genotype). Once introduced into the lab environment, bacteriophage T1 rapidly lyses
E. coli
strains that are commonly used in cloning applications and results in significant lab downtime and the loss of valuable clones. Bacteriophage T1 is particularly difficult to eliminate from the lab and can lay dormant for many years. The
tonA
genotype protects the phage T1-resistant TransforMax EC100-T1
R
cells and valuable clones from attack by bacteriophage T1.

Like the standard TransforMax EPI300
E. coli
, this strain has been specially engineered for use with Epicentre's CopyControl? Cloning Systems.* The cells contain an induc
IBL
e mutant
trfA
gene whose gene product is required for initiation of replication from the
ori
V contained on the CopyControl pCC1? Vectors or on clones that have been retrofitted with CopyControl cap
ABI
lity using the EZ-Tn5?<
ori
V/KAN-2> Insertion Kit. This process allows TransforMax EPI300-T1
R
E. coli
to maintain CopyControl vectors at single copy until induction occurs immediately before DNA purification.

Benefits

tonA
for resistance to bacteriophages T1 and T5.

trfA
gene under tight control of an induc
IBL
e promoter for copy-number control of CopyControl clones and clones retrofitted with the EZ-Tn5<
ori
V/KAN-2> Transposon.

High transformation efficiency of both large and small clones.

lacZΔM15
for blue/white screening of recombinants.

Re
ADI
ly accepts large DNAs for construction of large-insert genomic libraries.

Restriction minus [
mcrA, Δ(mrr-hsdRMS-mcrBC)
] enables efficient cloning of methylated DNA.

Endonuclease minus (
endA1
) to ensure high yields of DNA.

Recombination minus (
recA1
) for greater st
ABI
lity of large cloned inserts.

Genotype

F
-
mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ
-
rpsL (Str
R
) nupG trfA tonA

*Covered by issued and/or pending patents.


ORDER INFORMATION
1,000X concentrated solution. Filter sterilized.