商品编号


60215-1



品牌


Lucigen



公司


Lucigen



公司分类


CRISPR, Large or Difficult Fragment Cloning




10 rxns (Fivers)

商品信息


Cells that enable the most challenging library construction and screening.

Highest available transformation efficiencies for library construction and screening.

Optimized for high yields of DNA.





Lucigen
offers two bacterial strains designed for BAC library construction or transformation of large constructs:


BAC-Optimized Replicator? v2.0 Electrocompetent Cells
(> 1 × 10
10
cfu/?g pKanR DNA).? These cells are required for on-demand, copy number amplification of the pSMART? BAC or pEZ? BAC vectors in the CopyRight? Kits, thereby increasing DNA yields.? BAC-Optimized Replicator Cells are components of
Lucigen
’s
CopyRight
?
Cloning Kits
.


E. cloni
10G BAC-Optimized Electrocompetent Cells
(
>
1 × 10
7
cfu/?g 150 kb BAC DNA;
>
1 × 10
10
cfu/?g pUC DNA). The first competent cells developed by
Lucigen
specifically for large DNA cloning. ?Manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts, 10G BAC-Optimized Cells are an excellent choice for BAC library construction and large construct transformation.


Table 1.
E. cloni
Competent Cells for BAC or Large Fragment Cloning






E. cloni
Cell Lines



Transformation Efficiency
(cfu/?g pUC or pKanR DNA)




Cloning Methylated DNA




BAC, Cosmid Cloning




Blue/White Screening





10G BAC-Optimized Electrocompetent




>
1 × 10
10



YES



YES



YES
without IPTG induction





BAC-Optimized Replicator v2.0 Electrocompetent




>
1 × 10
10



YES



YES



YES
IPTG induction required







ORDER INFORMATION
Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube) or FIVERs (5 transformations per tube), as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

PLEASE NOTE:
Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all
Lucigen
Competent Cells. Please contact
Lucigen
.