商品编号


ASB71110



品牌


Lucigen



公司


Lucigen



公司分类


High Yield In Vitro Transcription Kits



Size

10 Reactions

商品信息

Produce micrograms of biotin-RNA or biotin-aRNA

Save time with 30 minute high-yield protocol, or extend the incubation to increase yield even further




Applications

Production of nonr
ADI
oactive RNA probes for any purpose, including microarray (transcription profiling) studies,
in situ
hybridization, and blotting experiments.

The AmpliScribe? T7-
Flash
? Biotin-RNA Transcription Kit enables high-yield synthesis of biotin-RNA or biotin-antisense RNA (aRNA, also called cRNA). The kit incorporates Biotin-16-UTP directly into the RNA transcript. High yields of biotin-RNA are produced from as little as 50 ng of input DNA template.

Features

Exceptionally high yields. The kit uses Epicentre's AmpliScribe T7-Flash rapid, high-yield
in vitro
transcription technology to generate the highest poss
IBL
e yield of biotin-RNA.

Optimized.
In vitro
transcription reaction conditions maximize yield of uniformly labeled biotin-RNA for high signal intensity.

Convenient. Rnase Inhibitor included.





Incubation Time (minutes)



30



60 (1 hour)



120 (2 hours)



480 (4 hours)



Template DNA



50 ng



5 - 10 ?g



15 - 25 ?g



30 - 40 ?g



60 - 70 ?g



100 ng



15 - 20 ?g



35 - 45 ?g



70 - 80 ?g



130 - 140 ?g



500 ng



60 - 70 ?g



140 - 150 ?g



> 180 ?g



> 180 ?g



1.0 ?g



125 - 135 ?g



> 180 ?g



> 180 ?g



> 180 ?g



Table 1. Yield of Biotin-RNA
(in mg) from varying amounts of control template DNA from a standard 37°C, 20 ml AmpliScribe T7-
Flash
Biotin-RNA Transcription Kit reaction over time. Results may vary depending on the template used.



ORDER INFORMATION
Contents: AmpliScribe? T7-Flash? Enzyme Solution, RiboGuard? RNase Inhibitor, AmpliScribe? T7-Flash? 10X Reaction Buffer, NTP/Biotin-UTP PreMix (ATP, CTP, GTP, UTP, and Biotin-16-UTP) Solution, RNase-Free Water, RNase-Free DNase I, DTT, Control DNA Template (linearized).