商品编号


ASF3257



品牌


Lucigen



公司


Lucigen



公司分类


High Yield In Vitro Transcription Kits



Size

25 Reactions

商品信息

Generate maximum amounts of RNA - over 180 ug RNA from 1 ug DNA template

Save time with 30 minute high-yield protocol

Scale up the reaction to produce grams of RNA

See FAQ




The AmpliScribe? T7-
Flash
? Transcription Kit uses Epicentre's rapid, high-yield
in vitro
transcription technology. An AmpliScribe T7-
Flash
Transcription reaction yields more RNA (180 ?g per 20-?l reaction) in 30 minutes than other high-yield
in vitro
transcription kit reactions produce in 2 hours (Fig. 1), including Epicentre's own AmpliScribe T7 High Yield Kit. The AmpliScribe T7-
Flash
Kit utilizes proprietary enzyme formulations that enable the maximum poss
IBL
e yields of RNA from virtually any template that is downstream of the T7 RNA polymerase promoter, including sequences in linearized plasmids, double-stranded oligos or PCR products.

Features

Exceptionally high yields. Up to 180 ?g of full-length RNA can be produced from 1 ?g of 1-kb linearized plasmid DNA template.

Scaleable. Reactions can be scaled up to obtain grams (or more) of RNA.

Fast. A standard AmpliScribe T7-
Flash
reaction is complete in 30 minutes.

Convenient. Rnase inhibitor included.

Trusted. Can be used for classic "Eberwine"-type RNA amplification procedures.

ORDER INFORMATION
AmpliScribe? T7-Flash? Enzyme Solution, AmpliScribe? T7-Flash? 10X Reaction Buffer, ATP, CTP, GTP and UTP Solutions, RNase-Free Water, RNase-Free DNase I, DTT, Control DNA Template (linearized), RiboGuard? RNase Inhibitor.