商品编号


MA-1001



品牌


Signosis



公司


Signosis



公司分类


miRNA Research




1 Ea


商品信息

Description:
miRNAs are known to involve in multiple
BIOLOG
ical functions and abnormal expression is associated to human diseases such as cancers. They represent a promising new class of cancer bio
Marker
s. A number of multiplex methods such as microarray or microbeads assays are commonly used for profiling the expression of multiple miRNAs simultaneously. However these methods need the conversion of the miRNA into a
CDN
A probe during analysis.
Signosis
has developed a simple miRNA direct hybridization plate array method, in which total RNA does not need any pre-treatment before hybridization. It is as simple as direct hybridization and detection. More importantly, total 48 miRNAs can be quantitatively analyzed and compared simultaneously with the sample assay.
Applicable Grid:
List of Applicable miRNAs

















?

1

2

3

4

5

6

7

8

9

10

11

12



A

let-7b-5p

miR-17-5p

miR-29a-3p

miR-132-3p

miR-182-5p

miR-206

let-7b-5p

miR-17-5p

miR-29a-3p

miR-132-3p

miR-182-5p

miR-206



B

miR-1

miR-18a-5p

miR-34a-5p

miR-133

miR-192-5p

miR-210-3p

miR-1

miR-18a-5p

miR-34a-5p

miR-133

miR-192-5p

miR-210-3p



C

miR-7-5p

miR-19a-3p

miR-92-3p

miR-143-3p

miR-194-5p

miR-214-3p

miR-7-5p

miR-19a-3p

miR-92-3p

miR-143-3p

miR-194-5p

miR-214-3p



D

miR-9-5p

miR-19b-3p

miR-101a

miR-145-5p

miR-196a-5p

miR-222-3p

miR-9-5p

miR-19b-3p

miR-101a

miR-145-5p

miR-196a-5p

miR-222-3p



E

miR-10b-5p

miR-20a-5p

miR-106a-5p

miR-146a-5p

miR-199a-5p

miR-223-3p

miR-10b-5p

miR-20a-5p

miR-106a-5p

miR-146a-5p

miR-199a-5p

miR-223-3p



F

miR-15a-5p

miR-21-5p

miR-107

miR-148a-3p

miR-200

miR-342-3p

miR-15a-5p

miR-21-5p

miR-107

miR-148a-3p

miR-200

miR-342-3p



G

miR-15b-5p

miR-24-3p

miR-122a-5p

miR-155-5p

miR-204-5p

miR-372-3p

miR-15b-5p

miR-24-3p

miR-122a-5p

miR-155-5p

miR-204-5p

miR-372-3p



H

miR-16-1

miR-26a-5p

miR-125a-5p

miR-181a-5p

miR-205-5p

U6

miR-16-1

miR-26a-5p

miR-125a-5p

miR-181a-5p

miR-205-5p

U6

Principle:
Signosis
’ miRNA direct hybridization plate array is a simple two-step assay; plate hybridization and streptavidin-HRP detection. The plate is pre-coated with an oligo mix, including a pair of unique oligos that hybridize side-by-side to a specific target miRNA and a universal capture oligo and a biotin-labeled oligo. In the assay, total RNA is directly utilized for hybridization. When the target miRNA exists in RNA, it acts as a bridge to bring the biotin-labeled oligo to the capture oligo, which can be detected through streptavidin-HRP conjugate and a chemiluminescent substrate. If there exists no specific miRNA, the biotin-labeled probe will be washed away, le
ADI
ng to no detection. In the plate array, 48 wells are coated with different oligo mixes for different miRNAs. A single 96-well plate allows quantitative measurement and comparison of 48 miRNAs between two samples. U6 RNA is used for normalization.

Data:

miRNA direct hybridization plate array I used for analyzing the expression of 48 miRNAs in human skeletal muscle and kidney total RNAs. As indicated in the figure, miR-1 and miRNA 133 are specifically expressed in skeletal muscle, which agrees with published studies.