商品编号


MA-1002



品牌


Signosis



公司


Signosis



公司分类


miRNA Research




1 Ea


商品信息

Description:
Apoptosis involves a series of biochemical events le
ADI
ng to a characteristic cell morphology and death, including changes to the cell membrane, cell shrinkage, nuclear fragmentation, chromatin condensation, and chromosomal DNA fragmentation. It plays important roles in removing unwanted cells during development and homeostasis. Excessive apoptosis causes hypotrophy, such as in ischemic damage, whereas an insufficient amount results in uncontrolled cell proliferation, such as cancer. MicroRNAs are newly discovered small non-coding RNA molecules that regulate up to 30% of mammalian gene expression. Numerous miRNAs have been revealed to have an influence on the regulation of apoptotic cell signaling such as overexpression of miR-145, miR-216, miR-182, and miR-96 miRNA to decrease caspase-3 activation.
Signosis
developed a miRNA array for targeting 47 miRNAs that have been shown in literature to be involved in apoptosis. Profiling the expression of these miRNAs will facilitate in uncovering the regulation of miRNA in apoptosis.
Applicable Grid:
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List of Applicable miRNAs

















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1

2

3

4

5

6

7

8

9

10

11

12



A

Let-7a-5p

miR-16-5p

miR-142

miR-155-5p

miR-202-5p

miR-337

Let-7a-5p

miR-16-5p

miR-142

miR-155-5p

miR-202-5p

miR-337



B

Let-7b-5p

miR-21-5p

miR-144

miR-182-5p

miR-204-5p

miR-338

Let-7b-5p

miR-21-5p

miR-144

miR-182-5p

miR-204-5p

miR-338



C

Let-7c-5p

miR-24-3p

miR-145-5p

miR-184

miR-210-3p

miR-345

Let-7c-5p

miR-24-3p

miR-145-5p

miR-184

miR-210-3p

miR-345



D

miR-1

miR-25-3p

miR-148

miR-188-5p

miR-214-3p

mir-376c-3p

miR-1

miR-25-3p

miR-148

miR-188-5p

miR-214-3p

mir-376c-3p



E

miR-7-5p

miR-26

miR-150-5p

miR-193a

miR-216-5p

miR-369-5p

miR-7-5p

miR-26

miR-150-5p

miR-193a

miR-216-5p

miR-369-5p



F

miR-10a-5p

miR-96

miR-151-3p

miR-193b

miR-218-5p

miR-369-3p

miR-10a-5p

miR-96

miR-151-3p

miR-193b

miR-218-5p

miR-369-3p



G

miR-15a-5p

miR-101

miR-152

miR-196a-5p

miR-224-5p

miR-371

miR-15a-5p

miR-101

miR-152

miR-196a-5p

miR-224-5p

miR-371



H

miR-15b-5p

miR-133b

miR-153-3p

miR-197-3p

miR-296-5p

U6

miR-15b-5p

miR-133b

miR-153-3p

miR-197-3p

miR-296-5p

U6

Principle:
Signosis
’ miRNA direct hybridization plate array is a simple two-step assay; plate hybridization and streptavidin-HRP detection. The plate is pre-coated with an oligo mix, including a pair of unique oligos that hybridize side-by-side to a specific target miRNA and a universal capture oligo and a biotin-labeled oligo. In the assay, total RNA is directly utilized for hybridization. When the target miRNA exists in RNA, it acts as a bridge to bring the biotin-labeled oligo to the capture oligo, which can be detected through streptavidin-HRP conjugate and a chemiluminescent substrate. If there exists no specific miRNA, the biotin-labeled probe will be washed away, le
ADI
ng to no detection. In the plate array, 48 wells are coated with different oligo mixes for different miRNAs. A single 96-well plate allows quantitative measurement and comparison of 48 miRNAs between two samples. U6 RNA is used for normalization.

Data:




Apoptosis-associated miRNA plate array used for analyzing the expression of 48 miRNAs in human HEK293 adn U937 total RNAs.


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