NEB/Bst 2.0 WarmStart® DNA Polymerase/M0538S/1,600 units分子诊断
New England Biolabs
PCR, qPCR & Amplification Technologies
2.0 WarmStart DNA Polymerase is an
designed homologue of
DNA Polymerase I, Large Fragment (
DNA Polymerase, Large Fragment) with a revers
y-bound aptamer, which inhibits polymerase activity at temperatures below 45°C. The aptamer rapidly releases the
2.0 WarmStart DNA Polymerase above 45°C and therefore no special activation step is needed to activate the polymerase.
2.0 WarmStart DNA Polymerase contains 5´→3´ DNA polymerase activity and strong strand-displacement activity but lacks 5´→3´ exonuclease activity.
2.0 WarmStart DNA Polymerase displays improved amplification speed, yield, salt tolerance, and
lity compared to wild-type
DNA Polymerase, Large Fragment.
Benefits of Bst 2.0 WarmStart
Identical LAMP reactions were run either immediately after setup (solid line) or after a 2 hour incubation at 25 °C. Without the protection from Bst 2.0 WarmStart, this room temperature incubation results in variable LAMP performance. Bst 2.0 WarmStart provides more consistent amplification reaction and enables room-temperature and high-throughput setup.
2.0 WarmStart DNA Polymerase is prepared from an
strain that expresses the
2.0 DNA Polymerase protein from an induc
The following reagents are supplied with this product:
Store at (°C)
Isothermal Amplification Buffer Pack
Magnesium Sulfate (MgSO
Bst 2.0 WarmStart DNA Polymerase does not exhibit 3´→ 5´ exonuclease activity.Reaction temperatures above 70°C are not recommended.Bst 2.0 WarmStart DNA Polymerase cannot be used for thermal cycle sequencing or PCR.Specific reaction conditions will vary for differentisothermal amplification applications. For best results, use 1X Isothermal Amplification Buffer.