NEB/Bst DNA Polymerase, Large Fragment/M0275S/1,600 units分子诊断
New England Biolabs
PCR, qPCR & Amplification Technologies
DNA Polymerase, Large Fragment is the portion of the
DNA Polymerase protein that contains the 5´ → 3´ polymerase activity, but lacks 5´ →3´ exonuclease activity.
Isolated from a recombinant source
Sequencing through problematic secondarystructures
Supplied with 10X Reaction Buffer
Polymerase, Large Fragment is prepared from an
strain containing a genetic fusion of the
DNA Polymerase gene, lacking the 5´ → 3´ exonuclease domain, and the gene coding for
maltose binding protein (MBP). The fusion protein is purified to near homogeneity and the MBP portion of the fusion is cleaved off in vitro. The remaining polymerase is purified free of MBP (1).
The following reagents are supplied with this product:
Store at (°C)
Reaction Buffer Pack
Magnesium Sulfate (MgSO
Bst DNA Polymerase does not exhibit 3´→ 5´ exonuclease activity.100 µg/ml BSA or 0.1%Triton X-100 is required for long term storage.Reaction temperatures above 70°C are not recommended.Bst DNA Polymerase, Large Fragment cannot be used for thermal cycle sequencing or PCR.