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NEB/Bst DNA Polymerase, Large Fragment/M0275S/1,600 units

分子诊断
NEB/Bst DNA Polymerase, Large Fragment/M0275S/1,600 units


商品编号


M0275S-1,600 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


PCR, qPCR & Amplification Technologies




1,600 units


Concentration

8,000 units/ml

商品信息

Description:

Bst
DNA Polymerase, Large Fragment is the portion of the
Bacillus
stearo
Thermo
philus
DNA Polymerase protein that contains the 5´ → 3´ polymerase activity, but lacks 5´ →3´ exonuclease activity.
Highlights
Isolated from a recombinant source
Sequencing through problematic secondarystructures
Supplied with 10X Reaction Buffer
Product Source
Bst
 Polymerase, Large Fragment is prepared from an 
E. coli
 strain containing a genetic fusion of the 
Bacillus stearo
Thermo
philus
 DNA Polymerase gene, lacking the 5´ → 3´ exonuclease domain, and the gene coding for
 E. coli 
maltose binding protein (MBP). The fusion protein is purified to near homogeneity and the MBP portion of the fusion is cleaved off in vitro. The remaining polymerase is purified free of MBP (1).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
Thermo
Pol
® 
Reaction Buffer Pack
-20
10X
Magnesium Sulfate (MgSO
4
) Solution
-20
100 mM
Notes:
Bst DNA Polymerase does not exhibit 3´→ 5´ exonuclease activity.100 µg/ml BSA or 0.1%Triton X-100 is required for long term storage.Reaction temperatures above 70°C are not recommended.Bst DNA Polymerase, Large Fragment cannot be used for thermal cycle sequencing or PCR.

上一条 NEB/WarmStart® LAMP Kit (DNA & RNA)/E1700S/100 reactions  下一步 NEB/Bst 2.0 WarmStart® DNA Polymerase/M0538S/1,600 units

产品货号:650.4

650.4 ¥
11至15个工作日送达