Product introduction: Coronavirus is a type of positive-strand RNA virus with membrane vesicles and linear genome. It is divided into four genera of α, β, γ, and δ. The new coronavirus belongs to the genus β-coronavirus. Coronavirus contains at least four types of structural proteins: spike protein S (Spike Protein), envelope protein E (Envelope Protein), membrane protein M (Membrance Protein) and nuclear protein N (Nucleocapsid Protein). Among them, the S protein is located on the surface of the viral envelope and can bind to the host cell surface receptor Ace2. The spatial structure of S protein can be divided into two parts, S1 and S2. S1 contains the receptor binding region (RBD). Anti-Covid-19 Spike Protein Antibody is a humanized monoclonal antibody that specifically recognizes the spike protein of the new coronavirus, expressed in the 293 cell system. Antibody activity is verified by Elisa using recombinantly expressed S-ECD and S-RBD proteins and is suitable for the detection of new coronaviruses.
Features: Humanized Fc antibody, Stable nature.
Host: Mouse
Antibody type: Monoclonal
Concentration(OD280): 2mg/ml
Buffer:20mM PB,PH=7.4
Affinity: EC50 = 0.0087ug/ml
Epitope position: S1 (RBD) area
Purity: Electrophoretic purity> 95%
Applications: ELISA detection,colloidal gold,chemiluminescence,immunoenrichment,etc.
The recommended concentration is as follows (ELISA antigen coating concentration 0.5μg / ml):
Test items | dilute proportion |
ELISA | 1:5,000-1:50,000 |
Western Blot | 1:1,000-1:10,000 |
Storage conditions: Store at -20 ℃ to avoid repeated freezing and thawing.
产品货号:SLAB05