商品编号


AG-40T-0236-C025



品牌


Adipogen



公司


Adipogen



公司分类


Cancer



Size

25 ?g

商品信息


More Information



Product Details



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Two human ubiquitin monomers (Accession Nr. P0CG47) covalently linked through an isopeptide bond at the K29 residue of one ubiquitin molecule and the C-terminal glycine residue of another ubiquitin molecule.



Crossreactivity

Human



MW

~17kDa



Purity

≥95% (SDS-PAGE)



Concentration

1mg/ml



Formulation

Liquid. In sterile, deionized water.



Other Product Data


Use:
Formed with wild-type human recombinant ubiquitin and linkage-specific enzymes. Validated by Ub-AQUA, an LCMS-based technique that provides extremely accurate information on the composition of Poly-ubiquitin samples. Ideal for investigating ubiquitin-?binding proteins and as substrates for ubiquitin?-specific isopeptidases. Reaction conditions will need to be optimized for each specific application.
IMPORTANT:
Heating this product in SDS-?PAGE buffer or terminating reactions containing this product with heated SDS-?PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-?PAGE buffer + DTT at <40°c for="" 20="" minutes="" prior="" to="" gel="" electrophoresis.="">40°c>



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





With a predicted molecular weight of 17 kDa, di-ubiquitin is composed of two ubiquitin monomers that are covalently linked through an isopeptide bond, which typically form between a lysine residue of one ubiquitin molecule and the C-terminal glycine residue of another ubiquitin molecule. Each human ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse ubiquitin, respectively. Ubiquitin has seven lysine residues that can participate in the formation of poly-ubiquitin chains. The specific lysine residue used in ubiquitin conjugation is thought to determine the function of poly-ubiquitination in cellular processes such as protein degradation, signaling and trafficking.
Linkage-specific di-ubiquitin is a substrate for enzymes that cleave the isopeptide linkage between two ubiquitin molecules. It can also be used to investigate mechanism of binding and recognition by ubiquitin-activating (E1) or ubiquitin-conugating (E2) enzymes, deubiquitinating enzymes, ubiquitin ligases (E3), or other proteins that contain ubiquitin associated domains (UBAs) or ubiquitin-interacting motifs (UIMs).