商品编号


F90101TH-10



品牌


FormuMax



公司


FormuMax Scientific Inc



公司分类


Trans-Hi? DNA Transfection Reagent



Size

0.5 mL








商品信息

Trans-Hi? ?is a superior transfection reagent capable to compete against the le
ADI
ng products on the market. It is based on a proprietary polymer technology. It forms stable nanoparticles with DNA and rapidly releases the DNA molecules upon endocytosis into the cytoplasm. It is a broad spectrum transfection regent offering superior and reproduc
IBL
e transfection efficiency on the common cells such as Hela, CHO, HEK293 and many hard-to-transfect mammalian cells with minimal toxicity. Trans-Hi
??
is very cost-efficient and 1.0mL is sufficient for over 660 transfections in 24 well plates.

?

Key Features for Trans-Hi
TM


Excellent transfection for single DNA transfection or multi DNA co-transfection

Rapid onset and long-lasting transfection

Suitable for very long DNAs

Simple and easy to use transfection procedure

Robust and reproduc
IBL
e transfection

High level of recombinant protein production

Very cost efficient

Long shelf-life: 12 months


?

Specifications:







?

Product type:

In vitro
cell transfection reagent



?

Applications

Plasmid transfection, protein overexpression



?

Technique:?

Polymer-based DNA nanoparticles?



?

Cell type:

Established cell lines, primary cells, hard-to-transfect cells ? ? ? ? ? ? ? ? ? Adherent cells and s
USP
ension cells



?

Sample type:

Plasmid DNA



?

Serum compatibility:?

Yes



?

Product size:

0.5, 1.0 and 10x1.0 mL



?

Number of transfections

Up to 660 in 24-well plates with 1.0mL reagent ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? up to 330 transfections in 6-well plates



?

Shelf-life:

12 months or longer when store refrigerated (2-8°C)



?

Shipping condition:

Room temperature




?

Protocols?

? ? ?Trans-Hi?General Protocol For
In Vitro
Transfection in Mammalian Cells?

? ? ?Trans-Hi? - Protocol for Transfecting 293 and CHO S
USP
ension Cells?

? ? ?Trans-Hi? - Protocol for Generation of rAAV?

? ? ?
Trans-Hi?
?-
T
echnical Tips For Good
In Vitro
Transfection
?

?


Comparison study of?
In vitro
plasmid DNA transfection of Trans-Hi
TM
and Lipofectamine?
?
2000?
in HEK293T cells
?
with GFP vector using the manufacture recommended reagent/DNA ratio of 3:1 (?L/?g) and 0.5?g DNA per well?used for both products in
24 well plate 30 hours post transfection.

?

Trans-Hi?/DNA ratio (?L/?g) (HEK293T)


Study of the transfection efficiency as a function of transfection reagent/DNA ratio
indicating optimal tranfection efficiency achieved at Trans-Hi?/DNA ratio of 3:1 (?L/?g) (
HEK293T cells in?24 well plate 30 hours post transfection, 0.5ug DNA per well).

?


Head-to-head comparison?
Trans-Hi? against Lipofectamine 2000?
HEK293A cells with GFP vector (reagent/DNA ratio of 3:1(?L/?g, 0.5ug per well), 24 well plate 48 hours post transfection).

?






?

Trans-Hi?

Lipofectamine-2000



Time

Day 1

Day 2

Day 3

Day 1

Day 2

Day 3



HEK293A (%)

79.5

93.4

93.3

75.7

89.4

50.6




?


Comparison study of?
In vitro
?plasmid DNA transfection of Trans-Hi??and Lipofectamine?
?
2000?
in Hela
?cells?
with GFP vector ((reagent/DNA ratio of 3:1(?L/?g, 0.5ug per well),?24 well plate 48 hours post transfection).

?

Broad Transfection Spectrum for Mammalian Cell Types





Cell Lines



Efficiency (% GFP)





COS-7



85 - 90%





Hela



85 - 90%





HEK-293T



85-90%





NIH 3T3



75%





A549



75%





Hep3D



65-75%





SHEP



65-70%





CaCo2



60%





HepG2



70%





SKOV3



65%





MCF-7



65-70%





3T3-442A



35-40%





CHO



80-90%