商品编号


11668030



品牌


Gibco



公司


Thermo Fisher Scientific



公司分类


Transfection Reagents



Quantity

0.3 mL










商品信息

Description
Lipofectamine? 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and s
USP
ension cell lines. Researchers use Lipofectamine? 2000 Reagent for siRNA- and shRNA-based gene knockdown experiments, as well as for gene expression studies.
With Lipofectamine? 2000 Transfection Reagent, you'll get:
? Exceptional transfection efficiency in the broadest range of cell lines and the highest levels of recombinant protein expression (see table)
? Superior performance for co-transfection of siRNA and plasmid DNA
? Proven efficacy in the presence of serum—eliminates the need to change media following transfection
? Reliable performance for high-throughput applications
? The best choice for establishing stable cell lines
A high-performance transfection reagent for gene expression and gene silencing
Lipofectamine? 2000 Transfection Reagent works effectively with all common cell lines as well as with many challenging ones, and can be used in media with or without serum. For gene silencing, Lipofectamine? 2000 Transfection Reagent's high-efficiency transfections provide the high levels of gene knockdown needed to produce convincing results. Lipofectamine? 2000 Transfection Reagent is the number one choice for co-transfection, given its effectiveness for transfecting both siRNA and plasmid DNA. Lipofectamine? 2000 Transfection Reagent is easy to use—simply mix with nucleic acid and add to cell culture.
Ideal for high-throughput work
Simplicity and speed combined with high transfection efficiency make Lipofectamine? 2000 Transfection Reagent ideal for transient protein expression or high-throughput RNAi transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications.
For Research Use Only. Not for use in diagnostic procedures.
Figures
Figure 2 - Lipofectamine??? 2000 Transfection Reagent combined with Stealth??? RNAi result in efficient luciferase knockdown
Lipofectamine?? 2000 transfection example
GFP expression
Figure 5 - Transfect neuronal or stem cells and achieve great transfection efficiencies
Lipofectamine??? 2000 cell line performance
Plasmid transfection
Figure 3 - Partial list* of cell lines successfully transfected with Lipofectamine??? 2000 Transfection Reagent and observed transfection efficiencies
Lipofectamine?? 2000 transfection protocol
Specifications
Cell Type:
Established Cell Lines,?Hard-to-Transfect Cells,?Primary Cells,?Stem Cells
Sample Type (Specific):
Plasmid DNA,?RNAi Plasmids (shRNA, miR),?Synthetic siRNA
Transfection Technique:
Lipid-Based Transfection
Serum Compat
IBL
e:
Yes
High Throughput Compatibility:
High Throughput-Compat
IBL
e
Product Line:
Lipofectamine?
Product Size:
0.75 mL
Shipping Condition:
Approved for shipment at Room Temperature or on Wet Ice
Green Features:
Sustainable packaging
Contents & storage
Contains one vial (0.75 ml) Lipofectamine? 2000 Reagent. Store at 2–8°C. Do not freeze.