商品编号


CnT-PR-MSC



品牌


CellnTec



公司


CellnTec



公司分类


MSC



Size

1 Ea

商品信息




Click for enlarged image. CnT-PR-MSC medium provides class-le
ADI
ng adMSC proliferation rates and longevity.


The new CnT-Prime MSC medium offers class-le
ADI
ng culture of both bone marrow and
ADI
pose-derived MSCs.
The growth rates of
ADI
pose-derived MSC exceeds key competitors (see image at left), all without using plate coatings!

Cells growing in CnT-Prime MSC medium also demonstrate extended longevity, and remain strongly multipotent for at least 4 passages.

It can easily be upgraded for clinical applications. Contact us at scientist@
CellnTec
.com for further details and pricing.

ADI
pose-derived MSCs grown in CnT-PR-MSC medium are CD45-/CD31-/CD73+/CD90+/CD105+/CD34+/-, and importantly at least 10% of the cell population remains CD34-positive at passage 4.


Figure:
CD34 expression in adMSC, CnT-PR-MSC medium, passage 4. Analysis courtesy of Dr Sean Hall, Dept. of Clinical Research, University Hospital Bern.

Cells may at any time be switched to the xeno-free #CnT-PR-MSC-XF medium, or induced to differentiate following addition of the necessary differentiation supplements.

For recommended isolation, expansion, differentiation and CFE protocols, please visit the Protocols Section of this website.

Like to try a test sample? Click here for more information.


CnT-Prime MSC Medium
CnT-Prime MSC Medium is a highly refined medium for high efficiency isolation and expansion of
ADI
pose-derived MSC. It uses an advanced basal medium supplemented with a range of growth factors, plus a low level of FCS.


Species
Tested with human cells, may be used with other species as well.
Tissue type
MSC
Pack size
500 mL bottle, fully supplemented with growth factors. No further additions required.
Product use
Developed specifically for optimal isolation efficiency and proliferation of MSCs isolated from bone marrow and
ADI
pose tissue. Recommended seeding density of adMSC immediately after isolation: 20'000 cells per sq. cm. Recommended seeding density of adMSC after passaging: 2'000 cells per sq. cm. See the Protocols Page for culture protocols.
Media Type
2D-Prolif
Defined
No
ACF
No
Culture condition
This medium is designed for use in a 5% CO
2
atmosphere. For suggested seeding and culture protocols, please visit the resources section of www.
CellnTec
.com
Note
For routine cell cultivation,
CellnTec
does not recommend the use of antibiotics / antimycotics.
Storage / Shelf life
Store frozen below -15
o
C. For best before date, see label. To prepare medium for use, it is preferable to thaw overnight at 4
o
C. Alternatively, medium may be thawed at room temperature for approx 5-6 hours. If thawing at room temperature, ensure contents do not rise above 12
o
C at the end of the thawing process. Once thawed, medium has a remaining shelf-life of 6-weeks when stored at 4
o
C in the dark. Certain culture media components are very sensitive to light. Minimize light exposure at all times.
Quality control
Media composition is tested via osmolality, pH and the concentration of various ions. Media functionality is tested by evaluating growth and morphology or primary human adMSC over at least 2 passages. Free of bacteria, fungi and mycoplasma contamination.
Shipping condition
Medium is shipped frozen.
Intended use
For research use only. Not for use in therapy or diagnostics.
Last update
2017-09-27