商品编号


4892-010-K



品牌


Trevigen



公司


Trevigen, Inc



公司分类


Apoptosis



Size

1000 Wells

商品信息

Description

Calcein AM is a non-fluorescent, hydrophobic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein, a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown, preferably in black-walled plates, can be stained and quantified in less than two hours.

Features:


Suitable for proliferating and non-proliferating cells.

Ideal for both s
USP
ension and adherent cells.

Non-r
ADI
oactive assay.

Rapid (no solubilization step as in a conventional MTT assay).

Ideal for high-throughput experiments.

Better retention and brightness compared to other fluorescent compounds (e.g. fluorescein).

Useful in a variety of studies, including: cell adhesion, chemotaxis, multidrug resistance, cell vi
ABI
lity, apoptosis, and cytotoxicity.

Adaptable to a wide variety of techniques, including: microplate assays, immunocytochemistry, flow cytometry, and in vivo cell tracking.


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Catalog # 4892-010-K includes :



Catalog Number

Description

Qty



4892-010-01

Calcein AM

2



4892-010-02

10X Calcein AM DW Buffer

1




Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions
Lija Swain, Marieke Wottawa, Annette Hillemann, Angelika Beneke, Haruki Odagiri, Kazutoyo Terada, Motoyoshi Endo, Yuichi Oike, Katja Farhat, and D?rthe M. Katschinski
J. Leukoc. Biol., Sep 2014; 96: 365 – 375.
http://www.jleukbio.org/cgi/content/abstract/96/3/365