商品编号


3457-096-02



品牌


Trevigen



公司


Trevigen, Inc



公司分类


Cell Based Assays



Size

1 ml

商品信息

Description

Cultrex? Bovine Collagen I?is purified from fetal bovine extensor?tendons?and?is pepsin-treated to remove telopeptides. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37? C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of 1 mg/mL.

Cultrex? 5X?Collagen I Solution is a purified stromal ECM protein?that has been developed, produced and qualified for the Cultrex? Collagen I Cell Invasion Assays.

Quality control specifications:


No bacterial or fungal growth detected after incubation at 37°C for 14 days following
USP
sterility testing guidelines.

Endotoxin concentration ≤ 20 EU/ml by Limulus Amoebocyte Lysate (LAL) assay.

No mycoplasma contamination detected by PCR.

Protein concentration of 1 mg/ml.

Provided in?20 mM Acetic Acid, pH 3.4 – 3.6.

Collagen I forms a gel when diluted to 0.4 mg/ml at neutral pH.

BIOLOG
ical activity determined for each lot:

Cell Attachment: Tested for the
ABI
lity to promote cell attachment and spre
ADI
ng of HT1080 human fibrosarcoma cells.

Performance tested in Cultrex? Collagen I Cell Invasion Assay.