商品编号


ax0325



品牌


Axolbio



公司


Axolbio



公司分类


Human Cells




1 Ea

商品信息

Description:
Human iPSC-Derived Neural Stem Cells that have been genetically edited using CRISPR-Cas9 technology to introduce the P301L mutation (CCG>CTG) into the
MAPT
gene. This line is heterozygous for the P301L mutation where one allele contains the mutation and the other allele is wild type at the locus. Click on the product images to see the data and further details.

The P301L mutation MAPT has been implicated in frontotemporal dementia and parkinsonism (Dumanchin
et al.
, 1998). The mutation affects only the 4R isoforms of MAPT since the exon containing the mutation is spliced out of 3R isoforms (Hutton
et al.
, 1998). Aggregated MAPT/tau protein in affected patients consisted mainly of the 4R isoform (Hutton
et al.
, 1998). The P301L mutation promotes aggregation of MAPT/tau protein into ordered paired helical filaments and beta sheet formation in vitro (von Bergen
et al.
, 2001).

Click here to see more data on Axol Neural Stem Cells
Product Specification:


Starting material

Dermal fibroblast



Donor gender

Female



Donor age at sampling

64 yrs



Karyotype

Normal



Reprogramming method

Episomal vector



Induction method

Monolayer & chemically defined medium



Genetic modification

Heterozygous for the MAPT P301L mutation (CCG>CTG)



Genetic modification

Contains a puromycin resistance cassette (intronic)



Size

≥1.5 million cells



Kit components

1 vial of Neural Stem Cells (≥1.5 million cells) and 1 bottle of Neural Plating-XF Medium (30 mL)



Growth properties

Adherent



Shipping conditions

Dry ice



Storage conditions

Liquid nitrogen