商品编号


R1920



品牌


Sciencell



公司


Sciencell research lab



公司分类


Hematopoietic Cell System




1 Ea

商品信息
Isolated from adult rat bone marrow tissue. Cells are harvested after purification and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume.

Macrophages are cells differentiated from circulating bone marrow-derived monocytes. In the bone marrow and subsequestly in the blood and tissues, monocytes undergo a cascade of maturation that culminate in morphologically and functionally mature tissue macrophages [1]. Macrophages function to removal cellular debris and inv
ADI
ng pathogens. They phagocytize inv
ADI
ng microorganisms, and scavenge dead, damaged cells and cellular debris. Macrophages can be identified by the specific expression of several cell surface proteins including CD14, CD11b, F4/80 (mice)/EMR1 (human), MAC-1/MAC-3, and CD68 by flow cytometry or immunohistochemical staining [2].

RMa-bm from ScienCell Research Laboratories are isolated from adult CD? IGS rat bone marrow. RMa-bm are harvested at P0 and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. RMa-bm are characterized by immunofluorescence with antibody specific to F4/80. RMa-bm are negative for mycoplasma, bacteria, yeast, and fungi. RMa-bm are guaranteed to further culture under the conditions provided by
Sciencell
Research Laboratories; however,
RMa-bm are not recommended for expanding or long-term cultures since the cells do not proliferate in regular culture.

Recommended Medium

It is recommended to use Macrophage Medium (MaM, Cat. #1921) for the culturing of RMa-bm
in vitro.

Specifications:
Catalog No.
R1920
Country of Manufacture
United States
Product Code
RMa-bm
Size/Quantity
1 x 10^6 cells/vial
Product Use
RMa-bm are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.
Shipping Info
Dry ice.
References
[1]. Martin Cline and Margaret Sumner (1972) Bone Marrow Macrophage Precursors. I. Some Functional Characteristics of the Early Cells of the Mouse Macrophage Series Blood 40(1) 62-69. [2]. Siamon Gordon and Philip Taylor (2005) Monocyte and macrophage heterogeneity. Nature Reviews Immunology 5(12) 953-964.