商品编号


M7540



品牌


Sciencell



公司


Sciencell research lab



公司分类


Human Pluripotent Stem Cells




1 Ea

商品信息
Isolated from embryonic day 13 CD1 mouse embryos. These cells are cryopreserved at primary culture and delivered frozen. Each vial contains 1 x 10^6 cells in 1 ml volume.

Mouse Embryonic Fibroblasts (MEF) are used to support the growth of mouse and human pluripotent stem cells [1]. MEF not only provide a substrate for pluripotent stem cells to grow on, but also secrete critical growth factors to maintain stem cell pluripotency. MEF are isolated from mouse embryos and used at early passages [2]. To serve as feeder cells, MEF must be treated with mitomycin C or by irr
ADI
ation to prevent cell proliferation. The treated cells can also be used to generate conditioned medium for feeder-free culture of pluripotent stem cells.

MEF from
Sciencell
Research Laboratories are isolated from embryonic day 13 CD1 mouse embryos. These cells are cryopreserved at primary culture and delivered frozen. Each vial contains 1 x 10^6 cells in 1 ml volume. MEF are characterized by immunofluorescence with antibody specific to fibronectin. MEF are negative for mycoplasma, bacteria, yeast, and fungi. MEF are guaranteed to further expand for 5 population doublings under the conditions provided by
Sciencell
Research Laboratories.

Recommended Medium

It is recommended to use DMEM (Cat. #09221) supplemented with 10% fetal bovine serum (FBS, Cat. #0010, 0025, 0500) for culturing MEF
in vitro
.

Specifications:
Catalog No.
M7540
Country of Manufacture

Product Code
MEF
Size/Quantity
1 x 10^6 cells/vial
Product Use
MEF are used as a feeder layer in mouse and human pluripotent stem cell culture. They are forresearch use only. They are not approved for human or animal use, or for application in in vitrodiagnostic procedures..
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen andkeep the cells in liquid nitrogen until they are needed for experiments.
Shipping Info
Dry ice.
References
[1] BRADLEY, A. (1987). Production and analysis of chimaeras. In Teratocarcinomas and Embryonic Stem Cells: A Practical Approach, (ed. E. J. Robertson), pp. 113-151. Oxford: IRL Press. [2] Nagy et al. (2006) Preparing Mouse Embryo Fibroblasts Cold Spring Harbor Protocols. 2006: pdb.prot 4398.